Fig. 5. Dependence of probabilities of stop codon appearance (pSC) (A), and of nonstop frame appearance (pNSF) on sense strands (open circles) and antisense strands (closed circles) upon GC content of a gene. The pNSF values, which are composed of 100 codons, were obtained by calculation with the pSC values on the strands, which were estimated from base compositions at three codon positions. Similar results were obtained by analyses of acidic and basic amino acid contents (data not shown).